Title
Identification of multiple transcripts for antioxidant protein 2 (Aop2): Differential regulation by oxidative stress and growth factors
Document Type
Article
Publication Date
2003
Abstract
Antioxidant protein 2 is a unique member of the thiol-specific antioxidant family of proteins known to reduce reactive oxygen species in the presence of thiol-containing electron donors. It is also a candidate atherosclerosis susceptibility gene in mice. In the present study, we sought to characterize the transcripts of this gene, and determine which, if any, are regulated by conditions associated with oxidative stress. We have identified multiple Aop2 transcripts by Northern blot, each exhibiting a unique tissue distribution. These include the previously reported 1.47 kb major transcript, two alternative Aop2 transcripts found exclusively in liver, and a testis-specific transcript believed to be the highly related intronless gene Aop2-rs1. Treatment of a murine hepatocyte cell line with glucose oxidase led to the specific and transient induction of the 1.47 kb transcript, while the 3.1 kb transcript was regulated by serum deprivation and re-stimulation with either keratinocyte growth factor or serum in a time-dependent manner. Since these ROS-inducing stimuli involve different mechanisms of action and cellular responses, our data suggest that alternative Aop2 transcripts may play distinct roles in different oxidative stress responses, and possibly in atherosclerosis.
Publication Title
Redox Report
Repository Citation
Sparling, N. and Phelan, Shelley A., "Identification of multiple transcripts for antioxidant protein 2 (Aop2): Differential regulation by oxidative stress and growth factors" (2003). Biology Faculty Publications. 14.
https://digitalcommons.fairfield.edu/biology-facultypubs/14
Published Citation
*Sparling N & Phelan SA. (2003) Identification of multiple transcripts for antioxidant protein 2 (Aop2): Differential regulation by oxidative stress and growth factors. Redox Report 8(2):87-94. *denotes student author
DOI
10.1179/135100003125001404
Peer Reviewed
Comments
© Maney Publishing, 2003. This is a post print of an article published in Redox Reports (8, 2, 2003). The publisher version is available at http://maney.co.uk/